The ROCK Inhibitor Y-26732 Enhances the Survival and Proliferation of Human Embryonic Stem Cell-Derived Neural Progenitor Cells upon Dissociation | Semantic Scholar (2024)

The results suggest that, in contrast to other stem cell types, Y-27632 supplementation is not a suitable strategy to enhance hADSC culture expansion.

A robust method for producing human mesencephalic DA neurons from hiPSCs is described and it is demonstrated that expanded mFPPs have increased DA neuron potential and differentiate more efficiently and rapidly than progenitors generated by standard protocols.

Human neural progenitor cells (hNPCs) support H5N1 virus infection and may play a role in the neuroinflammation during acute viral encephalitis, according to morphological observation and immunofluorescence.

It is demonstrated that exogenous basic fibroblast growth factor (bFGF) could be omitted from the hPSC culture media if the authors used the suitable feeder cells, and it was shown that although hPSCs can proliferate on the feeder-free culture system, however, genetic instability has been reported in such environment.

A robust, optimized monolayer procedure to rapidly convert hiPSCs into enriched populations of motor neuron progenitor cells (MNPCs) that can be further amplified to produce a large number of cells to cover many experimental needs is presented.

The data indicate that cell line‐specific differences dominate the variables that underline the differentiation efficiency in the early stages of differentiation, and EB generation method and maintenance conditions determine the later differentiation and maturation of retinal organoids.

The present study demonstrated the anti-degenerative effect of melatonin against DMSO- and ZIKV-induced degeneration in porcine iNSCs.

It is demonstrated that exogenous basic fibroblast growth factor (bFGF) could be omitted from the hPSC culture media if the authors used the suitable feeder cells, and it was shown that although hPSCs can proliferate on the feeder-free culture system, however, genetic instability has been reported in such environment.

iDAn are a promising and relevant in vitro cell model for studying cellular dysfunctions in PD pathology, and the phenotypic analysis of A53T SNCA and S NCA triplication iDAn enabled the detection of novel pathological mechanisms associated with PD.

The results demonstrate that the extracellular matrix-modified PAA-ACA gels support mouse and human neuronal cell attachment throughout the differentiation and maturation stages (14 and 28 days, respectively) and emphasize the utility of optimizing numerous biophysical cues for improved neuronal yield in vitro.

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The study implies that the numbers of prostate cells with stem/progenitor activity may be underestimated based on currently employed assays, supports that dissociation-induced apoptosis is a common feature of embryonic and somatic stem cells with an epithelial phenotype, and highlights the significance of environmental cues for the maintenance of stem cells.

It is concluded that conventional slow freezing with Y-27632 treatment is efficient and convenient for the cryopreservation of hES cells.

Application of a selective Rho-associated kinase (ROCK) inhibitor, Y-27632, to hES cells markedly diminishes dissociation-induced apoptosis, increases cloning efficiency and facilitates subcloning after gene transfer, and enables SFEB-cultured hES Cells to survive and differentiate into Bf1+ cortical and basal telencephalic progenitors.

Results indicate that inhibition of Rho/ROCK signaling may improve survival of grafted cells in cell replacement therapy and suggest that dissociation of neural precursor cells elicits an intrinsic pathway of cell death that is at least partially mediated through the Rho-ROCK pathway.

In conclusion, inhibition of the Rho‐associated kinase (ROCK) by Y‐27632 improved survival of dissociated hESC‐derived differentiated cells and facilitated development of applications of hESCs‐derived cardiomyocytes in multiple research areas.

The application of Y-27632 to hESCs after cell sorting improves cell recovery with no observed effect on pluripotency, and enables the consistent recovery of hESC by FACS using multiple surface markers.

This novel method for the cryopreservation of dissociated hESCs may reduce the time required to amplify frozen stocks, and facilitate not only the storage of large numbers of hESC but also the widespread use of these cells in regenerative medicine.

Increased thaw-survival and retention of stemness and differentiation potential in hWJSCs following cryopreservation is useful for their storage in cord blood banks for future regenerative medicine purposes.

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